Alternative Splicing (AS) Dynamics in Dwarf Soybean Derived from Cross of Glycine max and Glycine soja

Short crop height is the preferred breeding trait since there a positive correlation between lodging resistance and yield increase. Alternative splicing can alter transcriptome diversity contribute to plant adaptation environmental stress. We characterized transcriptomes obtained from dwarf normal soybean lines derived cross of Glycine max var. Peking (G. max) G. soja IT182936 in an F7 RIL population study differences isoforms. Full-length mRNA leaf tissues was sequenced using PacBio RSII platform, generating 904,474 circular consensus sequence (CCS) reads. Using Structural Quality Annotation Novel Transcript Isoforms (SQANTI) process, 42,582 44,762 high-quality isoforms, 91 179 polished low-quality isoforms were cells, respectively. As result, 832 36,772 nonredundant transcripts generated. Approximately 30% identified genes estimated produce two or more detected average 166,171 splice junctions (SJs), which 93.8% canonical SJs. that novel accounted for 19% all among 12% fell within coding regions. The demonstrated greater number most annotated genes, particularly related growth hormones defense responses. Our provides comprehensive isoform gene information may accelerate research provide basis further impact these on growth.